Serveur d'exploration Phytophthora

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Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors.

Identifieur interne : 002925 ( Main/Exploration ); précédent : 002924; suivant : 002926

Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors.

Auteurs : T. Eulgem [Allemagne] ; P J Rushton ; E. Schmelzer ; K. Hahlbrock ; I E Somssich

Source :

RBID : pubmed:10469648

Descripteurs français

English descriptors

Abstract

Parsley WRKY proteins comprise a family of plant-specific zinc-finger-type factors implicated in the regulation of genes associated with pathogen defence. In vitro, these proteins bind specifically to functionally defined TGAC-containing W box promoter elements within the Pathogenesis-Related Class10 (PR-10) genes. Here we present in vivo data demonstrating that WRKY1 is a transcriptional activator mediating fungal elicitor-induced gene expression by binding to W box elements. In situ RNA hybridization revealed that the WRKY1 gene is rapidly and locally activated in parsley leaf tissue around fungal infection sites. Transient expression studies in parsley protoplasts showed that a specific arrangement of W box elements in the WRKY1 promoter itself is necessary and sufficient for early activation and that WRKY1 binds to such elements. Our results demonstrate that WRKY transcription factors play an important role in the regulation of early defence-response genes including regulation of WRKY1.

DOI: 10.1093/emboj/18.17.4689
PubMed: 10469648
PubMed Central: PMC1171542


Affiliations:


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Le document en format XML

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<term>Amino Acid Sequence (MeSH)</term>
<term>Apiaceae (genetics)</term>
<term>Apiaceae (metabolism)</term>
<term>Base Sequence (MeSH)</term>
<term>DNA-Binding Proteins (metabolism)</term>
<term>Gene Deletion (MeSH)</term>
<term>Gene Expression Regulation (MeSH)</term>
<term>Gene Library (MeSH)</term>
<term>Humans (MeSH)</term>
<term>In Situ Hybridization (MeSH)</term>
<term>Models, Biological (MeSH)</term>
<term>Models, Genetic (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mutagenesis (MeSH)</term>
<term>Phytophthora (chemistry)</term>
<term>Plant Proteins (genetics)</term>
<term>Plasmids (MeSH)</term>
<term>RNA, Messenger (analysis)</term>
<term>Sequence Homology, Nucleic Acid (MeSH)</term>
<term>Time Factors (MeSH)</term>
<term>Transcription Factors (genetics)</term>
<term>Transcription Factors (metabolism)</term>
<term>Transcriptional Activation (MeSH)</term>
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<term>ARN messager (analyse)</term>
<term>Activation de la transcription (MeSH)</term>
<term>Apiaceae (génétique)</term>
<term>Apiaceae (métabolisme)</term>
<term>Banque de gènes (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Délétion de gène (MeSH)</term>
<term>Facteurs de transcription (génétique)</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Facteurs temps (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Hybridation in situ (MeSH)</term>
<term>Modèles biologiques (MeSH)</term>
<term>Modèles génétiques (MeSH)</term>
<term>Mutagenèse (MeSH)</term>
<term>Phytophthora (composition chimique)</term>
<term>Plasmides (MeSH)</term>
<term>Protéines de liaison à l'ADN (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Régulation de l'expression des gènes (MeSH)</term>
<term>Similitude de séquences d'acides nucléiques (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
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<term>Transcription Factors</term>
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<term>Transcription Factors</term>
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<term>Phytophthora</term>
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<term>Phytophthora</term>
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<term>Apiaceae</term>
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<term>Facteurs de transcription</term>
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<term>Apiaceae</term>
<term>Facteurs de transcription</term>
<term>Protéines de liaison à l'ADN</term>
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<term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Gene Deletion</term>
<term>Gene Expression Regulation</term>
<term>Gene Library</term>
<term>Humans</term>
<term>In Situ Hybridization</term>
<term>Models, Biological</term>
<term>Models, Genetic</term>
<term>Molecular Sequence Data</term>
<term>Mutagenesis</term>
<term>Plasmids</term>
<term>Sequence Homology, Nucleic Acid</term>
<term>Time Factors</term>
<term>Transcriptional Activation</term>
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<term>Activation de la transcription</term>
<term>Banque de gènes</term>
<term>Données de séquences moléculaires</term>
<term>Délétion de gène</term>
<term>Facteurs temps</term>
<term>Humains</term>
<term>Hybridation in situ</term>
<term>Modèles biologiques</term>
<term>Modèles génétiques</term>
<term>Mutagenèse</term>
<term>Plasmides</term>
<term>Régulation de l'expression des gènes</term>
<term>Similitude de séquences d'acides nucléiques</term>
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<div type="abstract" xml:lang="en">Parsley WRKY proteins comprise a family of plant-specific zinc-finger-type factors implicated in the regulation of genes associated with pathogen defence. In vitro, these proteins bind specifically to functionally defined TGAC-containing W box promoter elements within the Pathogenesis-Related Class10 (PR-10) genes. Here we present in vivo data demonstrating that WRKY1 is a transcriptional activator mediating fungal elicitor-induced gene expression by binding to W box elements. In situ RNA hybridization revealed that the WRKY1 gene is rapidly and locally activated in parsley leaf tissue around fungal infection sites. Transient expression studies in parsley protoplasts showed that a specific arrangement of W box elements in the WRKY1 promoter itself is necessary and sufficient for early activation and that WRKY1 binds to such elements. Our results demonstrate that WRKY transcription factors play an important role in the regulation of early defence-response genes including regulation of WRKY1.</div>
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